TB-500 (Thymosin Beta-4 fragment) is a synthetic peptide often used in the performance and recovery space. I have used TB-500 many times over the last several years and have found it effective for addressing injuries, building strength, and improving training recovery. It works particularly well when combined with BPC-157. Here’s a structured breakdown of its benefits based on available research and anecdotal reports:
🧬 Tissue Repair & Healing
- Accelerates wound healing – enhances cell migration and blood vessel formation in injured tissue.
- Speeds recovery from injuries – particularly tendon, ligament, and muscle tears.
- Promotes angiogenesis – new blood vessel formation improves nutrient and oxygen delivery.
💪 Musculoskeletal Benefits
- Tendon and ligament support – increases elasticity and repair rate of connective tissue.
- Muscle recovery – reduces downtime after heavy training or muscle strains.
- Joint health – may improve synovial healing and reduce stiffness.
🫀 Cardiovascular & Organ Protection
- Cardiac repair – studied for reducing damage after heart injury by promoting tissue regeneration.
- Organ healing – shows potential in liver and corneal repair in animal models.
🛡️ Anti-Inflammatory & Regenerative Effects
- Reduces inflammation – calms down excessive immune responses, aiding chronic injuries.
- Fibrosis prevention – may reduce scar tissue formation in muscles and organs.
- Neuroprotective effects – early studies suggest possible benefits in brain repair and stroke recovery.
⚡ Performance & Longevity Uses
- Injury prevention – some athletes use it preemptively to protect against wear-and-tear.
- Enhanced flexibility – by supporting actin cytoskeleton remodeling, it may improve tissue resilience.
- Systemic regeneration – general “healing acceleration” effect noted in anecdotal use.
Typical TB-500 Protocol
1. Loading Phase (Weeks 1–4)
- Dose: 4–5 mg per week
- Frequency: Split into 2 injections per week (e.g., Monday/Thursday)
- Route: Subcutaneous (SQ) or intramuscular (IM), typically near the injury site
Purpose: build up systemic levels quickly to accelerate healing.
2. Maintenance Phase (Weeks 5–8 or longer if needed)
- Dose: 2–5 mg every 1–2 weeks
- Frequency: Often 1 injection per week, tapering off gradually
- Duration: 4–6 weeks depending on injury recovery
Purpose: sustain tissue repair and prevent reinjury.
3. Combination Protocols
- With BPC-157 – common “healing stack”
- BPC-157: localized tendon/ligament healing
- TB-500: systemic healing, angiogenesis, tissue remodeling
- With Growth Hormone or peptides (e.g., GHRP-6, CJC-1295) – some users stack to enhance recovery.
- With anti-inflammatories (natural: curcumin, omega-3, boswellia) – synergistic in reducing chronic inflammation.
🧠 Injection Tips
- Rotate injection sites to prevent irritation.
- Many prefer subcutaneous administration (abdomen, thigh) for systemic effect.
- If targeting a specific injury (e.g., shoulder tendon), some go IM near the site.
✅ Best Use Case:
- Tendon, ligament, or muscle tear recovery
- Chronic inflammation that resists healing
- Adjunct to rehab and physiotherapy
🧬 Sample Healing Stack
Phase 1 – Loading (Weeks 1–4)
TB-500
- 4–5 mg per week, split into 2 injections (e.g., Mon/Thu)
- Route: Subcutaneous (abdomen/thigh) for systemic effect
BPC-157
- 250–500 mcg daily, injected near the injury site (subQ)
- Can be divided into AM/PM doses if preferred
Supplements
- Omega-3 fatty acids (2–3 g EPA+DHA daily) – reduces inflammation
- Curcumin (500–1000 mg/day with black pepper extract) – anti-inflammatory & collagen support
- Restroezyme 4 caps 2-3 times per day on an empty stomach
- Magnesium glycinate (300–400 mg nightly) – supports recovery and reduces cramping
- ASTB 2-3 caps to improve testosterone and growth hormone for enhanced healing
Phase 2 – Maintenance (Weeks 5–8+)
TB-500
- 2–5 mg every 1–2 weeks (tapering as healing improves)
BPC-157
- Continue 250 mcg daily, or reduce to 250 mcg every other day if recovery is advanced
Supplements
- Continue as above, may add:
- Boswellia serrata (500 mg twice daily) – additional joint protection
- Vitamin D3 + K2 (5000 IU + 100 mcg daily) – supports bone/tendon strength
Optional Enhancers
- GH secretagogues (CJC-1295 + Ipamorelin, or MK-677 orally) – may further accelerate tissue repair via IGF-1 signaling
- Red light therapy / PEMF – enhances mitochondrial recovery and blood flow
- Physiotherapy & mobility drills – critical for proper remodeling of healed tissue
⚠️ Safety Notes
- TB-500 and BPC-157 are research peptides — quality depends heavily on vendor.
- Always rotate injection sites to avoid irritation.
- More is not always better — excess angiogenesis stimulation (from TB-500) has unknown long-term risks.
- Stop or taper once healing is complete; these are meant for recovery, not indefinite use.
👉 This stack is widely used in the performance/recovery world because BPC-157 handles localized tendon/ligament healing, while TB-500 covers systemic regeneration and blood vessel growth, and the supplement base reduces inflammation and supplies building blocks for collagen repair.
Here are some notable research-grade TB‑500 vendors known for their quality and purity. These suppliers cater exclusively to research purposes and are not FDA‑approved or intended for human consumption.
Recommended Research-Grade TB-500 Vendors
I have used both of the options below and they are both high quality options. Make sure to sign up for their newsletters to get discount codes. They often have promotions with 15% off offers.
1. Peptide Sciences
- Renowned for producing peptides with >99% purity, verified through both HPLC and Mass Spectrometry. All synthesized under strict quality standards in the U.S
- Offers TB‑500 among a broad roster of scientifically validated peptides for research.
2. Core Peptides
- Provides TB‑500 with purity >99%, with Certificates of Analysis (COA) available on request
- Offers both 5 mg and 10 mg vials in lyophilized form, commonly used in lab settings.
Key Considerations for Choosing a Research Vendor
- Purity & QC: Opt for brands offering ≥99% purity with HPLC and/or Mass Spec verification.
- Documentation: Reliable COA or third-party lab reports are essential for verifying batch quality.
- Transparency: Check whether the vendor clearly labels their products as research-only and lists storage conditions, handling protocols, and compliance details.
- Regulatory Clarity: None of these peptides are approved for therapeutic use. Avoid using them outside of controlled research settings.
To assist with creating the right dosage, use the following peptide calculator
